Journal: Microorganisms

Article Title: Differences in the Shiga Toxin (Stx) 2a Phage Regulatory Switch Region Influence Stx2 Localization and Virulence of Stx-Producing Escherichia coli in Mice

doi: 10.3390/microorganisms11081925

Figure Lengend Snippet: The CI JH2010 , and CI JH2016 , and CI λ amino acid sequences and predicted protein structures. ( A ) Multiple sequence alignment of CI sequences by Clustal Omega from the JH2010 stx 2a -phage, JH2016 stx 2a -phage, and lambda phage. Amino acids highlighted in black show agreement with the reference CI JH2010 sequence. The black annotations below each sequence were determined by InterProScan, and represent the protein domains identified from the InterPro database. DNA-binding residues (blue) and the serine protease catalytic site residues (red) are also marked. ( B ) Tertiary protein structure predictions of CI JH2010 and CI JH2016 as determined by Phyre2 using CI λ as a reference (PDB #3BDN). The ribbon diagram is color-coded, based on secondary structure. All structures are shown with the C terminal region in the upper half of the image, and were oriented for viewing based on the noted N terminal helix (white arrow). Identified catalytic and DNA-binding residues were highlighted in the C and N terminal regions, respectively (yellow spheres).

Article Snippet: InterProScan (version 92.0) was used to analyze protein sequences for various protein domains within the InterPro database.

Techniques: Sequencing, Binding Assay